Ukugcina iimveliso zebhayoloji ezinobuthathaka ixesha elide, ibhotile ye-cryogenic Dewar yinkqubo ebonelela ngendawo ezinzileyo enobushushu obuphantsi ukugcina ubomi beeseli ezibuthathaka. I-Cryogenic Dewar luhlobo lwenqanawa engenalo uxinzelelo, eyilelwe ngokukodwa yenziwa, enokumelana nezinto ze-cryogenic ezinxulumene ne-nitrogen engamanzi. Initrojeni engamanzi ayinabumba, ayinambala, ayinangqondo, kwaye ayicaphukisi; Ke ngoko, ayinazimpawu zokulumkisa kwaye kufuneka iphathwe ngononophelo. Kumaqondo obushushu aphantsi ka-196 ℃, i-nitrogen engamanzi ithathwa njenge-cryogenic fluid, enokusetyenziselwa ukugcina izinto eziphilayo ezinomda.

Ngenxa yobukho be-nitrojeni engamanzi, ukucoca kwakhona kunokwenzeka. Ngolondolozo lwexesha elide lweeseli ezinamakhwiniba, izicwili kunye nezinye iisampulu kwiibhotile zeDewar ye-cryogenic, iinkqubo zonyango kunye nophando zinokuphinda zenziwe.

la manyathelo mahlanu alandelayo okukhusela i-cryogenic dewar kunye nemixholo yayo:

1. Sebenzisa inkqubo yokujonga ubushushu ethembekileyo. Ukuthintela nakuphi na ukusabela kwe-biochemical okunokubangela ukuthotywa kweseli, uninzi lweemveliso zebhayoloji ezibuthathaka kufuneka zigcinwe kumaqondo obushushu asezantsi kakhulu kwimibungu yecryogenic. Ubushushu obugciniweyo obugciniweyo (umzekelo, 196? C) bunokugcina ubomi bunomda kwizinto eziphilayo. Indlela efanelekileyo yokuqinisekisa ukhuseleko lomxholo weDewar kunye nokugcina ubushushu obuphantsi kukuphumeza inkqubo yokujonga ubushushu benitrogen.
3..Gcina iqondo lobushushu eliphantsi uDewar eme nkqo ngawo onke amaxesha. Umbethe weCryogenic kufuneka ugcinwe ume nkqo ngawo onke amaxesha ukuqinisekisa ukugcinwa okukhuselekileyo. Ukulahla i-dewar okanye ukuyibeka ecaleni kwayo kunokubangela ukuba i-nitrogen engamanzi iphuphume. Umonakalo kumdaka okanye nayiphi na into egcinwe kuwo inokwenzeka.
4..Akukho ukuphatha okuqatha. Ukuphathwa ngokurhabaxa kunokubangela umonakalo omkhulu kwiibhotile zangaphakathi ze-cryogenic Dewar kunye nemixholo. Yilahle ibhotile yeDewar, uyijike kwicala layo, kwaye ube nefuthe elibi kunye nokushukuma, okunokukhokelela ekuphulukaneni nenxalenye okanye kugqitywe ngokupheleleyo. Inkqubo yokugquma yokuthambisa inciphisa umthwalo wokudlulisa ubushushu kulwelo lwe-cryogenic kwaye igcina isiphithiphithi kubushushu obuphantsi ngalo lonke ixesha. Ubushushu obuzinzileyo obuphantsi bunokuhlangabezana namandla obushushu obufutshane.
5..Gcina isixhobo sicocekile kwaye somile. Isixhobo kufuneka sibekwe kwindawo ecocekileyo neyomileyo. Ukufuma, imichiza, izicoci ezinamandla kunye nezinye izinto zikhuthaza ukubola kwaye kufuneka zisuswe kwangoko. Coca nje ibhotile ye-cryogenic Dewar ngamanzi okanye isepha encinci kwaye yome kakuhle ukunqanda ukubola kweqokobhe lesinyithi. Ukonakaliswa kwezinto ezisetyenziselwa ukwenza ubumdaka kungabeka into egciniweyo emngciphekweni.
Gcina umoya owaneleyo. Ukungeniswa kwayo nayiphi na i-cryogenic Dewar akufuneki kugutyungelwe okanye kuthintelwe ukuthintela ukuphazamiseka ukuphuma kwegesi. I-Dewars ayicinezelwa, ke ukungabikho komoya ngokwaneleyo kungakhokelela kuxinzelelo lwegesi egqithisileyo. Oku kunokubangela ukuba ibhotile yeDewar iqhume kwaye ibe yingozi yokhuseleko lwabasebenzi kunye nezinto ezigciniweyo.